Disk Diffusion Method

∞ generated and posted on 2016.02.06 ∞

Means of determining antibiotic or disinfectant sensitivity that involves bacterial growth on agar-based media and small pieces of antibiotic- or disinfectant-impregnated filter paper.

See also zone of inhibition.

Disk-diffusion method for determining antibacteria sensitivity

Figure legend: Disks of filter paper are impregnated with antibiotic or disinfectant and then placed on an immature lawn of bacteria. The formation of bacteria-less zones of inhibition, and their diameter, serve as indications of sensitivity to the antibacterials employed.